Showing posts with label bioprinter. Show all posts
Showing posts with label bioprinter. Show all posts

Future Fabrication at MIT's Center for Bits and Atoms

If you are technology and science nerd you have to see this tour of MIT's Center for Bits and Atoms.

This is the place where some amazing tech is being developed for real world application purposes.
You will see some advanced gene editing, micron level 3d imaging, and manipulation, micron level laser cutters, molecular assemblers and nano machines.  


Part 1





Part 2




Some very very cool stuff kids!!!


MIT Center for Bits and Atoms homepage:

http://cba.mit.edu/

Source:

https://youtube.com/user/testedcom

Cytosurge FluidFM µ3Dprinter is world’s first sub-micron metal 3D printer

Cytosurge AG, based in Zurich Switzerland, presents their revolutionary FluidFM µ3Dprinter which is world’s first 3D sub-micron direct metal printing machine.
This 3D printer could be used as an advanced tool for development of many new scientific and engineering applications from biology to nanorobotics.
It is one of the first steps towards practical manufacturing of parts for nanobots floating in your body to repair the damaged cells.







From the video description:
At the forefront of nanotechnology, additive manufacturing, life sciences and single cell biology, Cytosurge FluidFM µ3Dprinter is the world’s first 3D printer capable of delivering sub-micron resolution in direct metal printing, while offering scalability and good prospects in both production cost and speed.
The FluidFM technology opens a new world for metal object manufacturing and enables research opportunities in fields such as microelectronics, semiconductors, surface modification, microbots, sensors, material science and many other fields. Virtually any design can be offered to the system, including overhanging structures with 90 degree angles, without support structures or post processing steps.
High-precision surface modification processes can also be executed by printing ultra-thin or structured objects and by depositing multiple metals at the target surface. With the FluidFM µ3Dprinter various metals like Cu, Ag, Au, Pt can be printed.
The printing of other metals (Sn, Cd, Cr, Ni etc.) and various alloys are under investigation.


Company homepage:

https://www.cytosurge.com/page/micro3dprinting



New Rapid Liquid Printing 3D Printing Process from MIT

Very smart people from MIT developed a novel 3d printing process called "Rapid Liquid Printing" where a material is injected into a gelatine cube medium that acts as a support. It increases the speed and you can get complex geometries.

You can see it in this video:





Process description:
In collaboration with Steelcase, we are presenting a new experimental process called Rapid Liquid Printing, a breakthrough 3D printing technology. Rapid Liquid Printing physically draws in 3D space within a gel suspension, and enables the creation of large scale, customized products made of real-world materials. Compared with other techniques we believe this is the first development to combine industrial materials with extremely fast print speeds in a precisely controlled process to yield large-scale products.
3D printing hasn’t taken off as a mainstream manufacturing process for three main reasons: 1) it’s too slow compared to conventional processes like injection molding, casting, milling, etc. 2) it’s limited by scale – although it’s good for creating small components, it’s not possible to produce large scale objects 3) the materials are typically low-quality compared to industrial materials.
Rapid Liquid Printing addresses all of these limitations: it is incredibly fast (producing structures in a matter of minutes), designed for large scale products (you can print an entire piece of furniture) and uses real-world, industrial-grade materials.

It looks interesting as a concept, but practicality is questionable. It takes a lot of gel support material, there are various foces, hard to design geometry due to the medium, the extruder "needle" effects the object geometry, materials need to be easy to separate... Still, it looks very promising for some future advanced applications and bioprinting.

MITs Self-Assembly Lab page:

http://www.selfassemblylab.net/

Detalied article on Dezeen:

https://www.dezeen.com/2017/04/28/mit-self-assembly-lab-rapid-liquid-printing-technology-produce-furniture-minutes-design/




OpenSLS Laser Sintering 3D Printer Made From Hacked Laser Cutter

Rice University bioengineering researchers have hacked a cheap Chinese CO2 laser cutter into a low(er)-cost laser sintering 3d printer and used it to produce fine objects such as liver structures. They claim it can be made under 10000 USD.
Materials used on it are nylon, sand, Candelilla wax, polycaprolactone (PCL) and other. 
Since there is a 40-80 W of laser energy, they use custom build inter gas module to prevent burining of the material.  

They have also made it open source, which deserves some gratitude from the community! Thank you Rice team! Sharing is caring!



























Here is the short presentation video:




Here is the powder distribution mechanism in action:



Project has a very detailed and extensive documentation well worth reading if you are interested in laser sintering and bioprinting.

GitHub with all the files:

https://github.com/MillerLabFTW/OpenSLS

Rice Uni news article:

http://news.rice.edu/2016/02/22/modified-laser-cutter-prints-3-d-objects-from-powder/

Ver detailed RepRapWiki page:

http://reprap.org/wiki/OpenSLS

Detailed PLOS research paper:

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0147399

If you want to make something similar, do keep in mind that lasers are dangerous, and nylon powder and simillar micro-powder materials are flammable and can also go KA-BOOM. Stay safe kids.

Here is the photo gallery:



Ourobotics Low-Cost DIY Renegade Open Source Bioprinter

Ourobotics is an Irish company that is developing bioprinters. They published an open source low cost bioprinter based on some standard RepRap parts named Renegade that can be sourced for some 900 USD. I doubt that someone will 3d print a kidney on it in a home workshop but some interesting biohacking project could come up in the future.




Ourobotics also developed high-end bioprinter that can print with 10 materials, has enclosed warm chamber for keeping the cells alive and costs some 12500 Euro.





... here it is printing an ear:




Ourobotics homepage:

https://www.weare3dbioprintinghumans.org/

PDF with build instructions based on a common RepRap:

http://www.3ders.org/images2016/ourobotics-bioprinter-instructions.pdf

More detailed article about the project:

http://www.3ders.org/articles/20160204-ourobotics-releases-completely-open-source-renegade-3d-bioprinter.html

3D Printed Silicone Nerve Repair Guide

Scientists developed a new 3d printing technology where they use printed silicone guides to regrow damaged nerves. It could help large number of disabled people.




Object description:
Specialized 3D printing is used to create a first-of-its-kind customized nerve regeneration guide with incorporated biochemical cues to promote both sensory and motor growth nerve regrowth. The red dots represent cues for motor growth the green dots represent cues for sensory regrowth. This research was led by University of Minnesota mechanical engineering professor Michael McAlpine.

More information:

http://nextbigfuture.com/2015/09/3d-printed-guide-helps-regrow-complex.html

http://onlinelibrary.wiley.com/doi/10.1002/adfm.201501760/abstract








Organic Structures in Gel Medium Made with Needle Toolhead




New and very precise technology comes out where fine needle is used to 3d print a sort of a intricate complex mold inside a gel cube. The mold can be used as a support for 3d printing organs or biological structures.

... and it just looks cool ...

Source and more info:

https://www.newscientist.com/article/dn28252-gel-scaffold-paves-way-for-3d-printing-of-biological-organs/

More science on it can be found at: http://advances.sciencemag.org/content/1/8/e1500655.full






High grade aluminum DIY syringe extruder by Liam Gilbertson

DIY syringe pumps and other syringe devices are used in different 3d printing projects for depositing various materials from living cells to solder paste dispensers.
Liam Gilbertson posted a very detailed tutorial on how to build your own syringe extrude from MendelMax 2.0 or any other 3D printer based on 2020 extrusion rails.
Be aware that this is not a novice level project since the extruder is very sturdy and may require some machining knowledge and more specialized tools like laser or water jet cutter able to cut 3mm aluminum.










Step-by-step build guide, all the files and code can be found here:

http://www.instructables.com/id/Build-a-Syringe-Extruder-for-your-MendelMax-20/

GitHub repository: https://github.com/LiamGilbertson/MM2SE


Free video webinar on 3d printing in medical industry

Here is a new free video webinar by Tyler Reid of GoEngineer on 3d printing in medical industry.
It is a great overview of current state of affairs in materials (types, certifications, features and sterilization), prototyping, fixtures, tooling, teaching aids, and production parts.
Well worth half an hour watching if you work in medical industry.





You can watch many more Tyler's webinars like:

http://diy3dprinting.blogspot.com/2014/12/free-webinar-on-3d-printing-and.html

http://diy3dprinting.blogspot.com/2014/07/free-webinar-of-3d-printed-end-use-parts.html


Biorep prototypes










EnvisionTEC high end 3d printers and bioplotter

I was just looking around what is industry standard and I found couple of videos from EnvisionTEC. They have some fine and expensive machines BUT as technology goes, one day you will have it on YOUR desktop.




... now, while you may have your workshop machine, most of you probably won't need a bioplotter since it is currently used as sophisticated medical instrument for special cases... or maybe DIY biohacking will explode. Making implantable 3d objects and body mods ... sounds like SF but we will see what future holds ...



http://envisiontec.com/3d-printers/3d-bioplotter/




3d printed human organs that generate electricity for medical implants from Uni of Iowa

Bioprinting is advancing rapidly, with many obstacles ahead, but at University of Iowa they are already planing to make "enhanced" 3d printed "superorgans" which also produce electricity that could be used for implanted (medical) devices.




Here is interview with Dr. Ibrahim Ozbolat, co-director of the University of Iowa's Advanced Manufacturing Technology Group.

Ozbolat told HuffPost Live's Caroline Modarressy-Tehrani that while current research is focusing on replacing failed organs, he's also interested in the prospect of developing a "brand new organ" that doesn't exist in nature but which could be transplanted to "enhance the functionality of the human body."
And he's thinking big. One possibility is an organ that generates electricity inside our bodies.
"For complicated organs -- for example, if the heart fails -- then you need a pacemaker. The pacemaker runs with batteries, and when the battery needs to be replaced, surgery is needed," he said. That procedure could be eliminated by creating "an organ that is going to be part of the human body and generate electricity that can run the heart."






















Source: HuffPo - http://www.huffingtonpost.com/2014/06/09/bioprinting-new-organ-electricity-video_n_5473949.html


There is still long way to go before fully functional organs

You will not be able to shoot lightning bolts from your hands anytime soon ... but one day ... one day ...


3d Bioprinting introduction, possibilities, problems and current state of technology





All you wanted to know about bioprinting and 3d printing with living cells summarized in 20 minute video. Must-watch if you are interested in the topic. Wei gives great overview of the field and current state of bioprinting technology which is truly amazing with incredible future possibilities (and problems to be solved).


Speaker: Prof. Wei Sun, Tsinghua University / Xin Innovation Workshop,

Presentation held at Tel Aviv University, 19 - 20.5.2014.

I found presentation by Prof. Wei Sun in PDF format similar to the one in the video which is great reference material on bioprinting:

http://nsfam.mae.ufl.edu/Slides/Sun.pdf

Conclusions are important and probably there are many scientists working on it right now:

In future we need the following to ensure 3d printed organs:
  • A new generation of biomaterials - Bio-Ink: go with cells (structure as cell delivery medium), grow with cells (support as cell ECM) and function with cells (as biomolecules); 
  • Developmental Engineering (vs. Developmental Biology) to fill the biological knowledge gap; 
  • Bio-3DP manufacturing tools: viable, reliable and reproducible, and capable of making heterogeneous structures; 
  • 4-D 3DP model: embedded time into Bio-3DP model: printing Stem Cells with control released molecules for complex tissues, Organs, Cellular Machines and Human-on-a-Chip devices



No, it is not a fruit 3d printer!

Hype machine is in action again with this "fruit 3d printer" contraption. Let us give some perspective to this:

  1. It doesn't print actual fruit
  2. Only "fruit" "printed" is a "raspberry" or to be more blunt, probably the only shape you can get from tiny spheres is raspberry. It is not even convincing raspberry. 
  3. It prints with tiny spheres of fruit paste or puree
  4. All the parts of the machine look as made from technology that exist for some time and it is open sourced (any 3d printer + syringe extruder like Cellstuder)

From the source:
Cambridge design company Dovetailed is today launching its 3D fruit printer, creating ‘fruit’ you can eat.
The company, which is working with Microsoft in Cambridge, says it takes just seconds to print an apple or a pear, or any other type of fruit.
The printer uses a molecular-gastronomy technique called spherification. It combines individual liquid droplets with different flavours into a fruit shape.
It is aimed at chefs, foodies and anyone interested in making creative dining experiences. No specialist knowledge of cuisine or molecular-gastronomy is required, and, the fruit produced is all organic.
On of the commentators got the point of the technology with the touch of sarcasm (aka. in the correct way):
"So you basically have to load it with apple in order to print an apple? Ingenious!"
The technology could be very useful for some specific purposes and niche markets but no one benefits from the marketing hype.

Source article with more information:

http://www.cambridge-news.co.uk/Business/Business-News/Unveiled-the-3D-printer-that-creates-fruit-you-can-eat-20140524070245.htm

Here is the company page:

http://www.dovetailed.co/

Fruit 3d printer. Nothing spectacular here. 

You can see the "3d printed fruit" on the spoon here. I've seen some fruit before, this doesn't look like it ...









Update:

Here is the video of Dovetailed "fruit 3d printer" in action. It still doesn't look like anything near fruit printing.



EnvisionTec launches new bioprinters for education and life sciences





New upgraded bioprinters are also emerging. The entire field of additive manufacturing is expanding. Imagine how many people will be saved one the technology is fully developed!

From the source article:
EnvisionTEC, a leading manufacturer of proprietary 3D rapid manufacturing solutions, announced today the launch of the newly re-engineered 3D-Bioplotter® for researchers. The Developer Series provides for the basic needs of Tissue Engineering and educational institutions who might not need all of the options available to the advanced operator using the 4th Generation Manufacturer Series.
The EnvisionTEC 3D-Bioplotter® has been used since 2000 for a variety of medical applications. Most research done to date using the 3D-Bioplotter® has been in the pre-clinical setting, yielding many publications by pre-eminent scientists from the materials science, imaging and toxicology disciplines. In the clinical setting, patient CT or MRI scans are used to create STL files to print solid 3D models which can then be used as templates for implants.
The EnvisionTEC 3D-Bioplotter® 3D printing technique may be described as the deposition of materials in three dimensions using air pressure. Materials range from polymer melts, through viscous pastes to liquids, and are inserted into syringes to be used in individual printing heads with individual needle tips. Air pressure is applied to the syringe, which then deposits a strand of material for the length of movement and time the pressure is applied. Parallel strands are plotted in each layer. In each layer, the direction of the strands is turned over the center of the object, creating a fine mesh with good mechanical properties and mathematically well-defined porosity. By permitting the use of pastes, hydrogels, melts, and any other liquid which may be quickly solidified, this technology enables a wide range of 3D printing applications.
With the addition of the Developer Series, the 3D-Bioplotter now comes in two versions to match the needs of varying users and budgets. CEO Al Siblani stated “The 3D-Bioplotter Developer Series is the ideal choice for the basic needs of educational institutions, while the 3D-Bioplotter® Manufacturer Series offers all options needed by advanced tissue engineering research or production.”
Source:

http://envisiontec.com/envisiontec-announces-launch-3d-bioplotter-developer-series-mrs-spring-meeting/



AJ TV report on printing replacment human skin for burn victims




From video description:
A new invention being put together could signal a major breakthrough in the way patients with burns injuries are treated. A researcher in Canada has developed a three-dimension prototype printer which will produce human skin from a patient's own cells.
Doctors say it will revolutionise the process of skin graft operations, and can save the lives of hundreds of burns victims every year.
And not just skin: the technology may also pave the way for producing entire organs for transplants.
Al Jazeera's Danel Lak reports from Toronto.

Source:

https://www.youtube.com/watch?v=UMwnC160Yrs


Cellstruder v2 with retractable syringe

Cellstuder is extruder that is design to deposit living cells in liquid form for DIY biology, biohacking or low-cost science purposes. Main advancement when compared to v1 is ability to retract the syringe with additional powered screw.





























Files and instructions:

http://www.thingiverse.com/thing:251647

Here is the overview of Cellstruder v1:

http://diy3dprinting.blogspot.com/2013/08/cellstruder.html

printGREEN 3d printer from Slovenia

Can you guess with what material is this printGREEN 3d printer from Slovenia printing?



The material is mixture of water, fertile soil and seeds. After some time, seeds sprout and plants start to grow. It is a sort of design art  project but I think this technology could be used in some agricultural or gardening machinery.

The team behind this project:Maja Petek, Tina Zidanšek, Urška Skaza, Danica Rženičnik and Simon Tržan. They worked with assistant professor Dušan Zidar to develop it. The original name in Slovenian is “Tiskaj ZELENO”. 
Project homepage:

http://tiskajzeleno.wix.com/tiskaj-zeleno#!printgreen/c4nw



























On personal note: Slovenians are our dear neighbors and I had several technology, art and DIY workshops with them. They have cool art-tech scene and are pretty chill crowd to hang out with.

Here is another cool project from Slovenia:

http://diy3dprinting.blogspot.com/2013/08/koruza-using-diy-3d-printing-for-laser.html

Update:

here are they at 3d Printshow in NYC, 2014:


3d printing blood vessels on a RepRap

Printing blood vessels out of sugar at Uni Pennsylvania lab.


From video description:
Bioengineers have been steadily advancing toward the goal of building lab-grown organs out of a patient's own cells, but a few major challenges remain. One of them is making vasculature, the blood vessel plumbing system that delivers nutrients and remove waste from the cells on the inside of a mass of tissue. Without these blood vessels, interior cells quickly suffocate and die.
Scientists can already grow thin layers of cells, so one proposed solution to the vasculature problem is to "print" the cells layer by layer, leaving openings for blood vessels as necessary. But this method leaves seams, and when blood is pumped through the vessels, it pushes those seams apart.
Bioengineers from the University of Pennsylvania have turned the problem inside out by using a 3D printer called a RepRap to make templates of blood vessel networks out of sugar. Once the networks are encased in a block of cells, the sugar can be dissolved, leaving a functional vascular network behind.
"I got the first hint of this solution when I visited a Body Worlds exhibit, where you can see plastic casts of free-standing, whole organ vasculature," says Bioengineering postdoc Jordan Miller.
Miller, along with Christopher Chen, the Skirkanich Professor of Innovation in the Department of Bioengineering, other members of Chen's lab, and colleagues from MIT, set out to show that this method of developing sugar vascular networks helps keep interior cells alive and functioning.
After the researchers design the network architecture on a computer, they feed the design to the RepRap. The printer begins building the walls of a stabilizing mold. Then it then draws filaments across the mold, pulling the sugar at different speeds to achieve the desired thickness of what will become the blood vessels.
After the sugar has hardened, the researchers add liver cells suspended in a gel to the mold. The gel surrounds the filaments, encasing the blood vessel template. After the gel sets it can be removed from the mold with the template still inside. The block of gel is then washed in water, dissolving the remaining sugar inside. The liquid sugar flows out of the vessels it has created without harming the growing cells.
"This new technology, from the cell's perspective, makes tissue formation a gentle and quick journey," says Chen.
The researchers have successfully pumped nutrient-rich media, and even blood, through these gels blocks' vascular systems. They also have experimentally shown that more of the liver cells survive and produce more metabolites in gels that have these networks.
The RepRap makes testing new vascular architectures quick and inexpensive, and the sugar is stable enough to ship the finished networks to labs that don't have 3D printers of their own. The researchers hope to eventually use this method to make implantable organs for animal studies.
Text by Evan Lerner
Video by Kurtis Sensenig

via: http://go3dprinting.tumblr.com/

http://www.upenn.edu/spotlights/rep-rap-3d-printing-blood-vessel-networks





BioPen repairs bones with handheld bioprinting

























From source page:
A handheld ‘bio pen’ developed in the labs of the University of Wollongong (UOW) will allow surgeons to design customised implants on-site and at the time of surgery.
The BioPen, developed by researchers from the UOW-headquarteredAustralian Research Council Centre of Excellence for Electromaterials Science (ACES), will give surgeons greater control over where the materials are deposited while also reducing the time the patient is in surgery by delivering live cells and growth factors directly to the site of injury, accelerating the regeneration of functional bone and cartilage.
The BioPen works similar to 3D printing methods by delivering cell material inside a biopolymer such as alginate, a seaweed extract, protected by a second, outer layer of gel material. The two layers of gel are combined in the pen head as it is extruded onto the bone surface and the surgeon ‘draws’ with the ink to fill in the damaged bone section.
A low powered ultra-violet light source is fixed to the device that solidifies the inks during dispensing, providing protection for the embedded cells while they are built up layer-by-layer to construct a 3D scaffold in the wound site.
Once the cells are ‘drawn’ onto the surgery site they will multiply, become differentiated into nerve cells, muscle cells or bone cells and will eventually turn from individual cells into a thriving community of cells in the form of a functioning a tissue, such as nerves, or a muscle.
The device can also be seeded with growth factors or other drugs to assist regrowth and recovery, while the hand-held design allows for precision in theatre and ease of transportation.
The BioPen prototype was designed and built using the 3D printing equipment in the labs at the University of Wollongong and was this week handed over to clinical partners at St Vincent’s Hospital Melbourne, led by Professor Peter Choong, who will work on optimising the cell material for use in clinical trials.
The BioPen will help build on recent work by ACES researchers where they were able to grow new knee cartilage from stem cells on 3D-printed scaffolds to treat cancers, osteoarthritis and traumatic injury.
Professor Peter Choong, Director of Orthopaedics at St Vincent’s Hospital Melbourne and the Sir Hugh Devine Professor of Surgery, University of Melbourne said:
“This type of treatment may be suitable for repairing acutely damaged bone and cartilage, for example from sporting or motor vehicle injuries. Professor Wallace’s research team brings together the science of stem cells and polymer chemistry to help surgeons design and personalise solutions for reconstructing bone and joint defects in real time.”
The BioPen will be transferred to St Vincent’s for clinical projects to be carried out at the proposed Aikenhead Centre for Medical Discovery in Melbourne.
“The combination of materials science and next-generation fabrication technology is creating opportunities that can only be executed through effective collaborations such as this,” ACES Director Professor Gordon Wallace said.
“What’s more, advances in 3D printing are enabling further hardware innovations in a rapid manner.”
Design expertise and fabrication of the BioPen was supported by the Materials Node of the Australian National Fabrication Facility, hosted at the University of Wollongong’s Innovation Campus.

Source:

http://media.uow.edu.au/news/UOW162803?utm_source=uow-homepage&utm_medium=main-banner-1&utm_campaign=news-biopen

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